João Lopes Perestrelo (II) died “in Espanhol” on May 5, 1606 (ANTT, Registos Paroquiais, Torres Vedras, Carvoeira, Mistos 1, fl. 49v), having determined in his will of 1587 that it would remain “in Chapel, and Morgado forever (…) This farmstead of mine of Ribeira do Hespanhol with all its belongings and the vineyard, olive oil press”. The new morgadio also included the lands in the Cintra term, called Murteira, parish of Igreja Nova, which he had inherited from his brother Miguel Nunes, and the houses that João Lopes Perestrelo had in Lisbon, “above the cross of the Catta que farás”, in addition to any other estates he owned at the time of his death. The morgadio‘s chapel was in the Igreja dos Mártires in Lisbon, where they had a tomb and where the founder intended to be buried (ANTT, Desembargo do Paço, Corte, Extremadura e Ilhas, mç. 1481, doc. 9).
Unmarried, João Lopes Perestrelo (II) named as heir, “as if by legitimate marriage”, the only son he had, António Perestrelo, born of a relationship with Maria Ferreira and legitimized by King Sebastião on May 5, 1578 (ANTT, Desembargo do Paço, Corte, Extremadura e Ilhas, mç. 1481, doc. 9). The new morgadio would be inherited by his descendants of legitimate marriage or, failing that, by bastards, always preferring males to females. If António Perestrelo had no descendants, António de Melo, nephew of the founder and son of his sister D. Filipa, would succeed him.
António Perestrelo, 1st administrator, died on December 8, 1621 and had married D. Luísa de Vasconcelos (who died on September 17, 1648), daughter of Paulo Dias da Fonseca and D. Maria Henriques de Vasconcelos. Through this marriage, several morgadios fell to the following generations of the Perestrelos do Hespanhol: Torre das Areias (1541), in Ervedal, near Avis; Bispo (1577), Pancas (1661), and Robert, all in Alenquer (MATOS, 1998: 62).